Publication Type: Edited Book
ADVANCES IN BIOTECHNOLOGICAL TOOLS AND TECHNIQUES FOR CROP IMPROVEMENT
Book Name: Futuristic Trends in Biotechnology Volume 3 Book 22
Authors: Gauri, Sood Suma n, Akash Sharma, Chander Kant
Keywords: Cisgenesis/transgenesis, CRISPR/CAS9, genome editing, New Biotechnological Tools (NBTs), RNA interference, zinc finger
Area/Stream: Biotechnology / Bioenergy / Others
Published in: IIP Series
Volume: 3, Month:May,Year:2024
Page No.: 163-186
e-ISBN: 978-93-6252-925-1
DOI/Link: https://www.doi.org/10.58532/V3BKBT22P3CH9
Abstract:
Biotechnology encompasses a suite of biological tools that have arisen from fundamental research and are currently utilized in both research and crop improvement. Modern plant breeding heavily relies on biotechnological methods to genetically enhance crops for desired traits and increased productivity. Traditional breeding techniques primarily rely on hybridization and the selection of advanced breeding lines. However, these conventional methods face challenges such as long juvenile phases, high levels of heterozygosity, and self-incompatibility. The introduction of foreign DNA into plants, achieved through methods like Biolistic transformation or Agrobacterium tumefaciens-mediated transformation, has played a pivotal role in genetic modification. Recent biotechnological advancements, particularly New Biotechnological Tools (NBTs) like RNA interference (RNAi), cisgenesis/intragenesis, trans-grafting, and genome editing techniques such as CRISPR/Cas9 and zinc-finger technology, now enable more precise and efficient genetic alterations in plants. These NBTs use genetic engineering to insert essential genes into the genomes of various plant species, resulting in improved efficiency and reliable genetic enhancements in clonally propagated plants while maintaining the stability of essential traits.
Cite this: Gauri, Sood Suma n, Akash Sharma, Chander Kant,"ADVANCES IN BIOTECHNOLOGICAL TOOLS AND TECHNIQUES FOR CROP IMPROVEMENT", Futuristic Trends in Biotechnology Volume 3 Book 22,IIP Series, Volume 3, May, 2024, Page no.163-186, e-ISBN: 978-93-6252-925-1, DOI/Link: https://www.doi.org/10.58532/V3BKBT22P3CH9